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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene is noticed in several tumors, which results in chromosome instability, and so might contribute to tumorigenesis. This protein was located to affiliate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a essential purpose in marketing cell-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have already been described. Two extra splice variants ended up documented but thorough nucleotide sequence data is just not still readily available.

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Blocking peptides are peptides that bind exclusively on the focus on antibody and block antibody binding. These peptide typically incorporates the epitope recognized via the antibody.

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This information outlines more possible optimization of cellular and immunoassay parameters to be certain the best possible success are...

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This tutorial outlines further achievable optimization of cellular and immunoassay parameters to guarantee the very best success are...

Antibodies certain to the blocking peptide no more bind to your epitope over the concentrate on protein. By evaluating the staining with the blocked antibody versus the antibody alone, you can see which staining is unique.

Concentrate on Facts Cyclin E belongs to your extremely conserved cyclin spouse and children, whose customers are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins function as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation patterns which add on the temporal coordination of each mitotic celebration. Cyclin E forms a fancy with and functions for a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S transition. This protein accumulates within the G1-S stage boundary which is degraded as cells progress by way of S phase.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...

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